(125)I-labeled recombinant human GAD65 and nonimmune human serum are incubated with the patient's diluted CSF. Antihuman IgG and IgM are then added to form an immunoprecipitate. After washing the precipitated immune complexes, specific antibodies are detected by counting gamma-emission from the pellet's bound (125)I-GAD65.(Walikonis JE, Lennon VA: Radioimmunoassay for glutamic acid decarboxylase [GAD65] autoantibodies as a diagnostic aid for stiff-man syndrome and a correlate of susceptibility to type 1 diabetes mellitus. Mayo Clin Proc 1998 December;73:1161-1166)
Monday to Friday; 6 a.m. and 4 p.m.
2 days/negative 3 days/positive