EEEVP - Performance: Red Blood Cell (RBC) Enzyme Evaluation

Test Catalog

Test ID: EEEVP    
Red Blood Cell (RBC) Enzyme Evaluation

Method Description Describes how the test is performed and provides a method-specific reference

Erythrocyte Enzyme Interpretation:

A hematopathologist who is an expert in these disorders evaluates the case, appropriate tests are performed and an interpretive report is issued.

 

Glucose-6-Phosphate Dehydrogenase (G6PD):

G6PD in a hemolysate catalyzes the oxidation of glucose-6-phosphate to 6-phosphogluconate. Concomitantly, nicotinamide adenine dinucleotide phosphate (NADP) is changed to its reduced form (nicotinamide adenine dinucleotide phosphate-oxidase: NADPH), a reaction measured spectrophotometrically.(Beutler E: Red Cell Metabolism: A Manual of Biochemical Methods. Third edition. New York, Grune and Stratton, 1984, pp 68-71)

 

Pyruvate Kinase:

A red cell hemolysate is incubated with adenosine diphosphate and phosphoenolpyruvate. The amount of pyruvate formed is quantitated by adding lactic dehydrogenase and reduced nicotinamide adenine di-nucleotide and measuring the rate of decrease in absorbance at 340 nm.(Beutler E: Red Cell Metabolism: A Manual of Biochemical Methods. Third edition. New York, Grune and Stratton, 1984, pp 68-71)

 

Glucose Phosphate Isomerase:

Washed erythrocytes are hemolyzed and the hemolysate is mixed with glucose, adenosine triphosphate (ATP), glucose-6-phosphate dehydrogenase, and nicotinamide adenine dinucleotide phosphate (NADP). The reduction of NADP is measured spectrophotometrically and is proportional to the enzymatic conversion of ATP and glucose to glucose-6-phosphate.(Beutler E: Red Cell Metabolism: A Manual of Biochemical Methods. Third edition. New York, Grune and Stratton, 1984, pp 40-42)

 

Hexokinase:

Hexokinase (in the presence of magnesium) catalyzes the reaction of ATP and glucose to G-6-P and ADP. In this assay the formation of glucose-6-phosphate (G-6-P) is measured by linking its further oxidation to 6-phosphogluconate (6-PG) to the reduction of NADP through the glucose-6-phosphate dehydrogenase (G-6-PD) reaction. The increase in absorbance which occurs as NADP+ is reduced is measured at 340 nm.(Beutler E: Red cell metabolism: A Manual of Biochemical Methods. Third edition. Grune and Stratton, New York, 1984, pp 38-40)

 

Glutathione:

Virtually all of the nonprotein sulfhydryl of red cells is in the form of reduced glutathione (GSH). 5,5'-dithiobis (2-nitrobenzoic acid) is a disulfide compound which is readily reduced by sulfhydryl compounds, forming a highly colored yellow anion. The absorbance of this resultant yellow substance is measured by 412 nm and compared to that of a known standard.(Beutler E: Red cell metabolism. In A Manual of Biochemical Methods, Second edition, Grune and Stratton, 1984)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2-10 days (not reported Saturday or Sunday)

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

13 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester