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Unit Code 8413:
Manganese, Serum

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Method Description

150 uL of each serum sample is diluted with 150 uL of reagent-grade

water and 2,700 uL of acidic diluent (1% HNO[3]) containing gallium,

cobalt, and yttrium internal standards. The diluted samples are then

vortexed and analyzed via dynamic reaction cell-inductively coupled

plasma-mass spectrometer (DRC-ICP-MS). The sample's raw

intensity is compared to a plasma matrix-matched calibration curve,

which spans from 0 ng/mL Mn spike to 10.90 ng/mL Mn spike

standards. Gallium, cobalt, and yttrium are combined as the internal

standard for manganese analysis, and the intensity of manganese

is adjusted according to the intensities of the internal standard.

(Unpublished Mayo method)

Performing Laboratory Location

Rochester

Key