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Dilutions of CSF are prepared and allowed to react with substrate
cells infected with the appropriate arbovirus. If antibodies to this
virus are present in the CSF of the patient, an antigen-antibody
complex will develop that can be detected by a fluorescein-labeled
antibody directed to human globulin. (Tsai TF: Arboviruses In
Manual of Clinical Microbiology. 7th edition. Edited by PR Murray,
EJ Baron, MA Pfaller, et al. Washington, DC, American Society for
Microbiology, 1999, pp 1107-1124; Beaty BJ, Casals J, Brown KL,
et al: Indirect fluorescent-antibody technique for serological
diagnosis of LaCrosse [California] virus infections. J Clin Microbiol
1982;15:429-434)

