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Unit Code 83873:
B-Type Natriuretic Peptide (BNP), Plasma

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Method Description

The instrument used is a Beckman Coulter DXI 800. The BNP test

is a 2-site immunoenzymatic sandwich assay. A sample is added

to a reaction vessel with mouse monoclonal antihuman BNP

antibody-alkaline phosphatase conjugate and paramagnetic

particles coated with mouse monoclonal antihuman BNP antibody.

BNP in human plasma binds to the immobilized anti-BNP on the

solid phase, while the mouse anti-BNP conjugate reacts specifically

with bound BNP. After incubation in a reaction vessel, separation in

a magnetic field and washing remove materials not bound to the

solid phase. The chemiluminescent substrate Lumi-Phos* 530 is

added to the reaction vessel and light generated by the reaction

is measured. The light production is directly proportional to the

concentration of BNP in the sample. The amount of analyte in the

sample is determined from a stored, multi-point calibration curve.

(Package insert:  Biosite Incorporated, France, 2003)

Performing Laboratory Location

Rochester

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