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Test ID: HISTO    
Histoplasma capsulatum Antibody Screen, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Polystyrene microwells are coated with inactivated Histoplasma antigen. Diluted serum samples and controls are incubated in the wells to allow antihistoplasma antibodies (if present in the sample) to react with the antigen. Nonspecific reactants are removed by washing and peroxidase-conjugated anti-human antibody that reacts with human antibodies bound to the antigen is added. Excess conjugate is removed by washing. Enzyme substrate and chromogen are added, and the color is allowed to develop. After adding the stop reagent, the resultant color change is quantified by a spectrophotometric reading of optical density (OD). Sample OD readings are compared with reference cutoff OD readings to determine results. (Package insert: Histoplasma DxSelect, Focus Diagnostics, Cypress, CA)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 3 p.m., Sunday; 9 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

2 weeks

Performing Laboratory Location The location of the laboratory that performs the test

Rochester