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Unit Code 83728:
Parietal Cell Antibodies, IgG, Serum

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Method Description

Purified H( )/K( ) ATPase antigen, isolated from pig gastric

mucosa, is bound to the wells of a polystyrene microwell plate

under conditions that will preserve the antigen in its native state.

Prediluted controls and diluted patient sera are added to separate

wells, allowing any H( )/K( ) ATPase antibodies present to bind to

the immobilized antigen. Unbound sample is washed away and an

enzyme-labeled antihuman IgG conjugate is added to each well.

A second incubation allows the enzyme-labeled antihuman IgG to

bind to any patient antibodies, which have become attached to the

microwells. After washing away any unbound enzyme-labeled

antihuman IgG, the remaining enzyme activity is measured by

adding a chromogenic substrate and measuring the intensity of the

color that develops. The assay can be evaluated spectrophoto-

metrically by measuring and comparing the color intensity that

develops in the patient wells with the color in the control wells.

(Package insert:  QUANTA Lite GPA, INOVA Diagnostics, Inc.,

December 2002, Revision 1)

Performing Laboratory Location

Rochester

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