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Unit Code 83727:
ZAP-70, Chronic Lymphocytic Leukemia (CLL) Prognosis

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Method Description

Ficoll-separated cells are initially stained with CD3 and CD19, then treated

with saponin and incubated with ZAP-70 antibody. Flow cytometry is

used to determine the percent of B-cells positive for ZAP-70. Staining

is also performed on a normal peripheral blood control specimen.

The threshold for ZAP-70 staining is established by using normal

B cells as the negative cutoff value and comparing with background-

positive T cell staining. (Rassenti LZ, Huynh L, Toy TL, et al:

ZAP-70 compared with immunoglobulin heavy-chain gene mutation

status as a predictor of disease progression in chronic lymphocytic

leukemia. N Engl J Med 2004 August 26;351[9]:893-901)

Performing Laboratory Location

Rochester

Key