LPSBF - Performance: Lipase, Body Fluid

Test Catalog

Test ID: LPSBF    
Lipase, Body Fluid

Method Description Describes how the test is performed and provides a method-specific reference

This is a colorimetric rate reaction. The lipase (LPS) method is an enzymatic method in which lipase catalyzes the hydrolysis of a natural 1,2-diglyceride liberating 2-monoglyceride and fatty acid. 2-monoglyceride is hydrolyzed by monoglyceride lipase to form glycerol and free fatty acid. Glycerol is then phosphorylated by glycerol kinase in the presence of adenosine triphosphate (ATP) to form glycerol-3-phosphate which is oxidized by glycerol-3-phosphate oxidase to form dihydroxyacetone phosphate and hydrogen peroxide (H2O2). Subsequently H2O2 reacts with 4-aminoantipyrine and sodium N-ethyl-N-(2-hydroxy-3-sulfopropyl)-m-toluidine in the presence of peroxidase to form quinone diimine dye. The dye absorbs light at 550 nm. The rate of increase in absorbance at 550 nm is directly proportional to the pancreatic lipase activity in the sample. The method is sensitive and specific for pancreatic lipase and utilizes colipase and deoxycholate as activators.(Sekisui Diagnostics Lipase reagent package insert, Charlottetown, PE, Canada)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday; Continuously

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

1 day

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test