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Test ID: ALS    
Aldolase, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Aldolase catalyzes the splitting of D-fructose 1,6-diphosphate into 2 molecules of triosephosphate, 1 of which is D-glyceraldehyde. The addition of triosephosphate isomerase converts the glyceraldehyde to dihydroxyacetone, which in turn is converted to alpha-glycerol phosphate by the further addition of alpha-glycerol phosphate dehydrogenase. This last enzymatic step also involves the conversion of NADH (reduced nicotinamide adenine dinucleotide) to NAD with a progressive loss of the characteristic absorbance of NADH at 340 nm. This conversion of NADH is measured kinetically.(Pinto PV, Kaplan A, Van Dreal PA: Aldolase. II. Spectrophotometric determination using an ultraviolet procedure. Clin Chem 1969;15:349-360)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Saturday; Continuously

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day (not reported on Sunday)

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester