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Unit Code 83391:
C4 Complement, Functional, Serum

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Method Description

C4 complement activity is measured by mixing patient serum

with a C4-deficient serum. The lytic activity of the serum mixture

is tested against sensitized, labeled liposomes. If lysis occurs, the

patient serum must be the source of the C4. The target liposomes

are a commercial reagent (WAKO total complement CH[50]), and

the assay is performed on a Hitachi 911. (Unpublished Mayo

information Yamamoto S, Kubotsu K, Masaaki K, et al: Automated

homogeneous liposome-based assay system for total complement

activity. Clin Chem 1995;41:586-590)

Performing Laboratory Location

Rochester

Key