|Values are valid only on day of printing.|
Muscle acetylcholine receptor (AChR) binding (IgG and IgM) are quantitated by immunoprecipitation assays. The high-affinity ligand (125)I-alpha-bungarotoxin is complexed with detergent-solubilized muscle AChR prepared from a mixture of innervated and denervated human muscle. AChR modulating antibody is detected in a bioassay: following 14-hour incubation with patient's serum, viable, noninnervated, monolayer cultures of human muscle cells are probed with (125)I-bungarotoxin to quantitate percent loss of surface AChR. Striational antibodies (IgG, IgM, and IgA) are detected by EIA using as antigen a mixture of sarcomeric proteins extracted from healthy adult rat skeletal muscle.(Griesmann GE, Kryzer TJ, Lennon, VA: Autoantibody profiles of myasthenia gravis and Lambert-Eaton myasthenic syndrome. In Manual of Clinical and Laboratory Immunology. Sixth edition. Edited by NR Rose, RG Hamilton, B Detrick, Washington, DC, ASM Press, 2002, pp 1005-1012)
CRMP-5-IgG is detected by Western blot assay using recombinant human collapsin response-mediator protein-5 as antigen.(Yu Z, Kryzer TJ, Griesmann GE, et al: CRMP-5 neuronal autoantibody: marker of lung cancer and thymoma-related autoimmunity. Ann Neurol 2001 February;49:146-154)
ACh receptor (muscle) binding antibody: Monday through Friday, Sunday; 2 p.m.
ACh receptor (muscle) modulating antibodies: Monday through Thursday, Saturday; 12 p.m.
Striational (striated muscle) antibodies: Monday through Friday; 2 p.m.
CRMP-5-IgG Western blot: Monday through Friday; 8 a.m.
AChR ganglionic neuronal antibody: Sunday through Thursday; 10 p.m.
Neuronal (V-G) K+ channel autoantibody: Sunday through Thursday; 10 p.m.
GAD65 antibody assay: Monday through Friday; 2 a.m.