|Values are valid only on day of printing.|
Calcium channel binding antibodies (IgG and IgM) are measured quantitatively by immunoprecipitation assays. Selective high-affinity ligands labeled with (125)I are complexed with cation channel proteins detergent-solubilized from membranes of pig cerebral cortical synaptic membranes; omega conopeptide GVIA identifies neuronal N-type Ca(++) channels; omega conopeptide MVIIC identifies neuronal P/Q-type Ca(++) channels; (125)I-alpha-bungarotoxin identifies muscle acetylcholine receptor (AChR) solubilized from a mixture of innervated and denervated human skeletal muscle. AChR modulating antibody is detected in a bioassay: following 14 hours of incubation with patient's serum, viable, noninnervated, monolayer cultures of human muscle cells are probed with (125)I-bungarotoxin to quantitate percent loss of surface AChR. Striational antibodies (IgG, IgM, and IgA) are detected by EIA using as antigen a mixture of sarcomeric proteins extracted from healthy adult rat skeletal muscle.(Griesmann GE, Kryzer TJ, Lennon VA: Autoantibody profiles of myasthenia gravis and Lambert-Eaton myasthenic syndrome. In Manual of Clinical and Laboratory Immunology. Sixth edition. Edited by NR Rose, RG Hamilton, B Detrick. Washington, DC, ASM Press, 2002, pp 1005-1012)
ACh receptor (muscle) binding antibody: Monday through Friday, Sunday; 2 p.m.
ACh receptor (muscle) modulating antibodies: Monday through Thursday, Saturday; 12 p.m.
Striational (striated muscle) antibodies: Monday through Friday; 2 p.m.
CRMP-5-IgG Western blot: Monday through Friday; 8 a.m.
AChR ganglionic neuronal antibody: Sunday through Thursday; 10 p.m.
Neuronal VGKC autoantibody: Sunday through Thursday; 10 p.m.
GAD65 antibody assay: Monday through Friday; 2 a.m.