Hepatitis A Total Antibodies, Serum
Method Description Describes how the test is performed and provides a method-specific reference
The VITROS antihepatitis A virus (anti-HAV) total assay is a competitive immunoassay method based on the preincubation of anti-HAV total in the sample with HAV antigen in the assay reagent, followed by incubation with horseradish peroxidase (HRP)-labeled antibody conjugate (mouse monoclonal anti-HAV). The immune complex is captured by streptavidin on the well surface. Unbound conjugate is removed by washing.
The bound HRP conjugate is measured by a luminescent reaction. A reagent containing luminogenic substrates (a luminol derivative and a peracid salt) and an electron transfer agent is added to the wells. The HRP in the bound conjugate catalyzes the oxidation of the luminol derivative, producing light. The electron transfer agent increases the level and duration of the light produced. The light signals are read by the VITROS Immunodiagnostic system. The amount of HRP conjugate is indicative of the concentration of anti-HAV present in the sample.(Package insert: VITROS Anti-HAV Total Reagent Pack, no. GEM1235A, version 4.0, Ortho-Clinical Diagnostics, Inc. Rochester, NY 14626-5101, 1/22/2012)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test