Multiple Sclerosis (MS) Profile
Method Description Describes how the test is performed and provides a method-specific reference
The oligoclonal banding (OCB) assay requires paired cerebrospinal fluid (CSF) and serum samples. Unconcentrated CSF and diluted serum are electrophoresed by isoelectric focusing. The separated Ig are visualized by an IgG immunoblot, and oligoclonal bands that are present in the CSF and not in the serum are reported. The assay uses reagents from Helena Laboratories. (Keir G, Luxton RW, Thompson EJ: Isoelectric focusing of cerebrospinal fluid immunoglobulin G: an annotated update. Ann Clin Biochem 1990 September;27:436-443)
The CSF IgG index also requires paired CSF and serum samples. The CSF and serum IgG and albumin are determined by immunonephelometry on a Siemens Nephelometer II. The CSF IgG index and synthesis rate are calculated and reported. In addition, the serum IgG and albumin, CSF IgG and albumin, and serum and CSF IgG/albumin ratios are reported. (Markowitz H, Kokmen E: Neurologic diseases and the cerebrospinal fluid immunoglobulin profile. Mayo Clin Proc 1983 April;58:273-274; Siemens Nephelometer II Operators Manual 1999)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; 7 a.m. - 12 p.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Stored serum and CSF: 2 weeks
Performing Laboratory Location The location of the laboratory that performs the test