Indirect immunofluorescence. Dilutions of test sera are prepared and allowed to react with substrate cells infected with the appropriate arbovirus. If IgG antibodies to this virus are present in the serum of the patient, an antigen-antibody complex will develop that can be detected by a fluorescein-labeled antibody directed to human globulin. (Tsai TF: Arboviruses. In Manual of Clinical Microbiology. Seventh edition. Edited by PR Murray, EJ Baron, MA Pfaller, et al. Washington, DC, American Society for Microbiology, 1999, pp 1107-1124; Beaty BJ, Casals J, Brown KL, et al: Indirect fluorescent-antibody technique for serological diagnosis of LaCrosse [California] virus infections. J Clin Microbiol 1982;15:429-434)
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