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Unit Code 83189:
Ganglioside Antibody Panel, Serum

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Method Description

The serum antibodies are detected by enzyme-linked immunosorbent

assay (ELISA) which is more specific and faster than immunofluor-

escence. These ganglioside antigens (GM1, etc.) are applied to wells

of an ELISA plate. Serially diluted patient's serum is added. After

washing, alkaline phosphatase conjugated antibodies to human

IgG and IgM are added, washing is repeated, then ezyme substrate

is added. Results are expressed as antibody titer, ie, the greatest

dilution at which the optical density (405) reading of the reaction

product is positive (values >2.0 times the mean value of 4 normal

sera are considered positive). (Pestronk A, Cornblath DR, Ilyas AA,

et al: A treatable multifocal motor neuropathy with antibodies to GM1

ganglioside. Ann Neurol 1988;24:73-78)

Performing Laboratory Location

Rochester

Key