|Values are valid only on day of printing.|
Busulfan analysis is performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with calculation of area under the curve (AUC) by integration of the time versus concentration curve from start of infusion to last specimen collection (6-hour AUC).(Mayo unpublished method)
AUC and clearance are calculated after quantification of busulfan concentration in blood plasma samples:
-1 specimen drawn immediately after termination of a 2-hour intravenous (IV) infusion of 0.8 mg/kg busulfan
-1 specimen drawn 1 hour after the infusion is terminated
-1 specimen drawn 2 hours after the infusion is terminated
-1 specimen drawn 4 hours after the infusion is terminated
The AUC is calculated by integration of the plot of time in minutes from the start of infusion until the last specimen collection (independent variable), with busulfan concentration in mcmol/L (dependent variable), and with straight-line extrapolation of the immediate postinfusion busulfan concentration to time zero. Clearance is calculated as the dose divided by AUC.
The recommended optimal dose is calculated based on the assumption of an ideal AUC=1100 mcmol*min.(Slattery JT, Risler LJ: Therapeutic monitoring of busulfan in hematopoietic stem cell transplantation. Ther Drug Monit 1998 October;20:543-549)
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