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N-methylhistamine (NMH) is extracted from urine using solid-phase extraction. The elute is analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and quantified using a stable isotope labeled internal standard.(Martens-Lobenhoffer J, Neumann HJ: Determination of 1-methylhistamine and 1-methylimidazole acetic acid in human urine as a tool for the diagnosis of mastocytosis. J Chromatogr B Biomed Sci Appl 1999;721:135-140)
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