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Unit Code 8301:
Respiratory Syncytial Virus (RSV) Antibodies, IgG and IgM (Separate Determinations), Serum

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Method Description

The indirect immunofluorescence test is used for the measurement

of IgG and IgM antibodies to RSV. Specific antibodies present in the

serum of the patient react with RSV infected cells that have been

previously fixed on a glass microscope slide. Fluorescein-labeled

anti-human IgG or IgM conjugate is used to stain specific antibody

bound to the substrate cells. The slides are examined with a

fluorescence microscope for the characteristic apple-green fluorescence

of the infected cells. (Urquhardt GE, Walker GH: Immunofluorescence

for routine diagnosis of respiratory syncytial virus infection.  J Clin

Pathol 1972;25:843-845)

Performing Laboratory Location

Rochester

Key