Epstein-Barr Virus (EBV), Molecular Detection, Quantitative PCR, Blood
Method Description Describes how the test is performed and provides a method-specific reference
Quantitative Epstein-Barr virus (EBV) DNA determinations are obtained by LightCycler PCR.
Viral DNA is extracted by the MagNA Pure automated instrument (Roche Applied Science) from whole blood. LightCycler PCR primers and probes have been designed to detect target EBV DNA. Amplified product is detected by a fluorescence assay (fluorescence resonance energy transfer [FRET]). Quantitative results are obtained by incorporating known copy levels (5 standards) of EBV DNA (plasmid) into the assay together with the sample. LightCycler instrument software calculates the level of EBV DNA (copy/mL) in the sample from a standard curve of the quantitative levels of the reference plasmid EBV DNA.(4,5) (Espy MJ, Patel R, Paya C, Smith TF: Quantification of Epstein-Barr virus viral load in transplant patients by LightCycler PCR. ASM Press 101(st) General Meeting [Abstract]. 2001;May 20-24)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Saturday; Varies
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Same day/1 day
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test