|Values are valid only on day of printing.|
Complement Fixation (CF):
Antibody to coccidioidin in the patient's serum is quantitated by CF. The CF test is a 2-stage test based on the ability of antigen-antibody complexes to bind complement (Cβ). In the first stage, antigen and antibody combine and fix Cβ. The second stage is an indicator system in which sheep erythrocytes, sensitized by rabbit anti-sheep red cell antibody (hemolysin), are used to demonstrate the presence of unfixed Cβ. If the patientβs serum contains Cβ-fixing antibody that reacts with the specific antigen (a positive reaction), Cβ will be fixed and excess Cβ will not be available to react with and lyse the sensitized sheep erythrocytes. If no antigen-antibody reaction occurs (a negative reaction), Cβ will be available to lyse the sheep erythrocytes. The CF titer is determined by the greatest dilution of serum (antibody) in which the sheep erythrocytes are not lysed.(Kaufman L, Kovacs JA, Reiss E: Immunomycology. In Manual of Clinical Laboratory Immunology. Fifth edition. Edited by NR Rose, ED de Macario, JD Folds, et al. Washington, DC, ASM Press, 1997 pp 591-592; Pappagianis D, Zimmer BL: Serology of coccidioidomycosis. Clin Microbiol Rev 1990;3:247-268)
ID is a qualitative test employed for the detection of precipitating antibodies present in the serum. Soluble antigens of the fungus are placed in wells of an agarose gel filled Petri dish and the patientβs serum and a control (positive) serum are placed in adjoining wells. If present, specific precipitate antibody will form precipitin lines between the wells. Their comparison to the control serum establishes the results. When performing the ID test, only precipitin bands of identity with the reference bands are significant.(Kaufman L, Kovacs JA, Reiss E: Immunomycology. In Manual of Clinical Laboratory Immunology. Fifth edition. Edited by NR Rose,EC de Macario, JD Folds, et al. Washington, DC, ASM Press, 1997, pp 591-593; Pappagianis D, Zimmer BL: Serology of coccidioidomycosis. Clin Microbiol Rev 1990;3:247-268)
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