Unit Code 82589:
T and B Quantitation, New York
Method Description
The T- and B-cell surface marker assay uses monoclonal antibodies to
identify the various membrane antigens, and flow cytometry to
enumerate the number of cells expressing these differentiation
antigens. The results are reported as the percent of lymphocytes
that are T-helper (CD3 , CD4 ), T-suppressor(CD3 ,CD8 ), natural killer
(CD16 CD56 , CD3-), and B-lymphocytes(CD19 ), and the absolute
number of each cell type per mL of blood. The assay is a 4-color
no-wash procedure and the absolute counts are calculated from
internal bead standards. In addition, the total lymphocyte count and the
helper-suppressor ratio (T[H]/T[S]) is reported. (Hoffman RA, Kung PC,
Hansen WP, Goedstien G: Simple and rapid measurement of human
T lymphocytes and their subclasses in peripheral blood. Proc Natl Acad
Sci USA, 77:4914-4917, 1980; U.S. Department of Health and Human
Services: Guidelines for performance of CD4 T-cell determinations in
persons with human immunodeficiency virus infection. MMWR 46 no.
RR-2: 1997, pp 1-29)
Performing Laboratory Location
Rochester
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