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Test ID: P1433    
14-3-3 Protein, Spinal Fluid

Method Description Describes how the test is performed and provides a method-specific reference

The 14-3-3 protein is measured in an immunochemiluminometric assay. The patient specimen is incubated with a monoclonal antibody directed against all 14-3-3 isoform coated on white microtiter plate wells. After washing, a second monoclonal antibody directed against the theta/tau isoforms, labeled with an acridinium ester is added. The amount of label subsequently bound to the wells is counted in a microtiter plate luminometer that calculates the amount of 14-3-3 present in the specimen.(Preissner CM, Aksamit AJ, Parisi JE, Grebe SK: Development and validation of an immunochemiluminometric assay for 14-3-3 protein. Clin Chem 2009;55[S6]: page A199; abstract D-149. Poster available on request)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday, Thursday; 1 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

6 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 months

Performing Laboratory Location The location of the laboratory that performs the test

Rochester