Mycobacterial Culture, Blood
Method Description Describes how the test is performed and provides a method-specific reference
Blood is drawn and must be transported to the laboratory (ambient) within 48 hours. The blood is processed per the manufacturerâ€™s instructions before adding it to a VersaTREK Myco bottle and plating onto Middlebrook 7H10 agar. The agar plate is incubated at 37 degrees C with 5% to 7% carbon dioxide (CO2) for 42 days. The VersaTREK Myco bottle is incubated on the automated VersaTREK 528 instrument for 42 days. If the bottle signals as positive on the instrument, it is removed and a smear is performed to look for acid-fast organisms. Acid-fast organisms are identified using conventional methods including nucleic acid hybridization probes, MALDI-TOF mass spectrometry, 16S rDNA gene sequencing.(Mirrett S, Hanson KE, Reller LB: Controlled clinical comparison of VersaTREK and BacT/ALERT blood culture systems. J Clin Microbiol 2007;45:299-302; Buckwalter SP, Olson SL, Connelly BJ, et al: Evaluation of MALDI-TOF Mass Spectrometry for the Identification of Mycobacterium species, Nocardia species and Other Aerobic Actinomycetes. J Clin Microbiol 2016; in press (doi:10.1128/JCM.02128-15): Hall L, Doerr KA, Wohlfiel SL, Roberts GD: Evaluation of the MicroSeq system for identification of mycobacteria by 16S ribosomal DNA sequencing and its integration into a routine clinical mycobacteriology laboratory. J Clin Microbiol 2003;41:1447-1453)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Continuously
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test