Urine proteins are separated in an electric field according to their size, shape, and electric charge (Helena SPIFE 3000). The separation is performed on agarose gels (Helena SPIFE SPE Vis Gel). The proteins are visualized by staining with acid blue and the intensity of staining is quantitated by densitometry (Helena Quick Scan 2000). Multiplying by the urine protein concentration (Coomasie blue) converts the percentage of protein in each fraction into urine concentration.(Instruction manual: Helena SPIFE 3000; package insert: Helena SPIFE SPE Vis Gel, 2001; Kyle RA, Katzmann JA, Lust JA, Dispenzieri A: Clinical indications and applications of electrophoresis and immunofixation. In Manual of Clinical Laboratory Immunology. Sixth edition. Edited by NR Rose, RG Hamilton, B Detrick: Washington, DC, ASM Press, 2002 pp 71-91)
Protein, total: Monday through Sunday; Continuously
Electrophoresis, protein: Monday through Saturday; 12 p.m.