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Unit Code 82441:
Electrophoresis, Protein, Urine

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Method Description

Urine proteins are separated in an electric field according to their

size, shape, and electric charge (Helena SPIFE 3000). The separation

is performed on agarose gels (Helena SPIFE SPE Vis Gel). The

proteins are visualized by staining with acid blue and the intensity

of staining is quantitated by densitometry (Helena Quick Scan 2000).

Multiplying by the urine protein concentration (Coomasie blue) converts

the percentage of protein in each fraction into urine concentration.

Performing Laboratory Location

Rochester

Key