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| Web: | MayoMedicalLaboratories.com |
|---|---|
| Email: | mml@mayo.edu |
| Telephone: | 800.533.1710 |
| International: | 507.266.5700 |
| Values are valid only on day of printing. | |
Urine proteins are separated in an electric field according to their
size, shape, and electric charge (Helena SPIFE 3000). The separation
is performed on agarose gels (Helena SPIFE SPE Vis Gel). The
proteins are visualized by staining with acid blue and the intensity
of staining is quantitated by densitometry (Helena Quick Scan 2000).
Multiplying by the urine protein concentration (Coomasie blue) converts
the percentage of protein in each fraction into urine concentration.

