Unit Code 82426:
Fatty Acid Profile, Essential (C12-C22), Serum
Method Description
Quantitation of fatty acids of specific chain lengths is performed as
follows. A 2-step, acid-base hydrolysis is followed by hexane extraction
and derivatization with pentafluorobenzyl bromide. Separation and
detection are accomplished by capillary gas chromatography
electron-capture negative ion-mass spectrometry. Quantitation is based
on analysis in the selected ion-monitoring mode by using 13 stable
isotope-labeled internal standards. (Lagerstedt SA, Hinrichs DR, Batt SM,
Magera MJ, et al: Quantitative determination of plasma C8-C26 total
fatty acids for the biochemical diagnosis of nutritional and metabolic
disorders. Mol Genet Metab 2001;73:38-45)
Performing Laboratory Location
Rochester
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