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Test ID: CDTA    
Carbohydrate Deficient Transferrin, Adult, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Transferrin is purified from serum using affinity chromatography. The chromatography is done using an antihuman transferrin antibody that has been coupled to POROS-aldehyde media. Serum is applied to the affinity column, which sequesters transferrin isoforms using pH 7.4 phosphate buffer saline. Transferrin isoforms (TrfI) are eluted from the affinity column with pH 2.5 100mM glycine/2% acetic acid buffer and concentrated on a C4 column, which is then washed with water/methanol/glacial acetic acid (97/2/1) to remove excess phosphate and other buffer components, which suppress mass spectrometer (MS) response. TrfI are eluted from the C4 column and introduced to the MS using methanol/ water/glacial acetic acid/TFA (94.5/5/0.5/0.04). The MS is operated in Q1 scan mode from 2,000 to 3,000 amu with a complete analysis time of 6.5 minutes. Relative quantitation of carbohydrate deficient transferring is achieved by comparing a-oligosaccharide transferrin and mono-oligosaccharide transferrin to di-oligosaccharide transferrin.(Lacey JM, Bergen R, Magera MJ, et al: Rapid determination of transferrin isoforms by immunoaffinity liquid chromatography and electrospray mass spectrometry. Clin Chem 2001;47:513-518)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Wednesday: 8 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

5 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

10 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 month

Performing Laboratory Location The location of the laboratory that performs the test

Rochester