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Test ID: AMKPK    
Amikacin, Peak, Serum

Method Description Describes how the test is performed and provides a method-specific reference

This assay is performed by enzyme-multiplied immunoassay technique (EMIT) using an Olympus analyzer: The EMIT assay is a homogeneous enzyme immunoassay technique used for the analysis of specific compounds in biological fluids. The assay is based on competition for antibody binding sites between drug in the specimen and drug labeled with the enzyme glucose-6-phosphate dehydrogenase (G6P-DH). Enzyme activity decreases upon binding to the antibody, so the drug concentration in the specimen can be measured in terms of enzyme activity. Active enzyme converts oxidized nicotinamide adenine dinucleotide (NAD) to NADH, resulting in an absorbance change that is measured spectrophotometrically. Endogenous serum G6P-DH does not interfere, because the coenzyme functions only with the bacterial enzyme employed in the assay. (Package insert: EMIT Amikacin Assay, Syva Company, Siemens Healthcare Diagnostics, Newark, DE, February 2007).

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Sunday; Varies

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

2 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester