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Test ID: PBGU    
Porphobilinogen, Quantitative, Random, Urine

Method Description Describes how the test is performed and provides a method-specific reference

Porphobilinogen (PBG) in urine is quantified by liquid chromatography tandem-mass spectrometry analysis in positive ion mode. An internal standard (13C2-PBG) is added to 1 mL of acidified urine. PBG and 13C2-PBG are extracted from the sample using an Oasis HLB column on a Gilson ASPEC Workstation. Separation of PBG from the specimen matrix is achieved by a reverse phase HPLC. PBG and the internal standard are monitored through their corresponding precursor and product ion pairs. The ion pair for PBG and 13C2-PBG are m/z 227 to m/z 210 and m/z 229 to m/z 212, respectively.(Ford RE, Magera MJ, Kloke KM, et al: Quantitative measurement of porphobilinogen in urine by stable-isotope dilution liquid chromatography-tandem mass spectrometry. Clin Chem 2001 September;47[9]:1627-1632)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 8 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days (not reported on Saturday or Sunday)

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

6 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location The location of the laboratory that performs the test

Rochester