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Unit Code 82026:
Narcolepsy Associated Antigen, Blood

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Method Description

The purpose of this procedure is to amplify target segments of

DNA within the Class II region of the human major histocompatibility

complex such that allelic variability corresponding to the serologically

defined specificities for DR subregions can be identified.  One of the

genomic regions common to most narcoleptic patients across all ethnic

groups is DQBeta1*0602.  Setup includes mixing the HotStart Taq

polymerase chain reaction (PCR) mix with the DNA specimen and

dispensing the mixture in a thermoplate containing primers and thermal

cycling.  After amplification, the PCR products are loaded onto an agarose

gel.  After electrophoresis, the ethidium bromide stained gel is then

photographed and interpretation is made.  (Moore SB:  Unpublished

Mayo information).

Performing Laboratory Location

Rochester

Key