|Values are valid only on day of printing.|
Calibrators, controls, and patient specimens are incubated overnight with I(125) 21-hydroxylase. Antibody, if present, binds to the 21-hydroxylase during this time. Protein A is added to precipitate the antibodies present, the assay tubes are centrifuged, and the pellet is counted. The amount of radioactivity in the pellet is directly proportional to the amount of antibody contained in the specimen.(Package insert: 21-Hydroxylase [21-OH] Antibody Kit. Kronus, San Clemente, CA)
Thursday; 2 p.m.