Search our Test Catalog. Specify what to search (name, test code, titles, everywhere) and how to search (begins with, contains)

Unit Code 81967:
Activated Protein C Resistance V (APCRV), Plasma

Print Friendly View
Print Page Email Page

Method Description

This assay is performed using the Chromogenix COATEST APC

Resistance V Kit on the ACL TOP. The method uses a modified

APTT test to detect APC-resistance. The plasma specimen is

prediluted in factor V-deficient plasma. Then the APTT test is

performed by incubating patient plasma with a standardized amount

of platelet-like phospholipids and activator of the contact factors of

the intrinsic coagulation pathway, followed by recalcification of

plasma and measurement of clotting time.

 

The ratio of the APTT test with and without added APC is reported as

the APC resistance (or sensitivity) ratio. (Kapiotis S, Quehenberger P,

Jilma B, et al:  Improved characteristics of APC resistance assay,

COATEST APC resistance by predilution of samples with factor V

deficient plasma. Am J Clin Pathol 1996;106:588-593; Fryburger G,

Javorschi S, Labrouche S, Bernard P:  Proposal for objective

evaluation of the performance of various functional APC-resistance

tests in genotyped patients. Thromb Haemost 1997;78:1360-1365;

Hall C, Andersson N, Andras M, et al:  Evaluation of a modified APTT-

based method for determination of APC resistance in plasma from

patients on heparin or oral anticoagulant therapy. Thromb Res 1998;89:

203-209)

Performing Laboratory Location

Rochester

Key