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Unit Code 81939:
Fatty Acid Profile, Mitochondrial (C8-C18), Serum

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Method Description

Quantitation of fatty acids of specific chain lengths is performed as

follows. A 2-step, acid-base hydrolysis is followed by hexane

extraction and derivatization with pentafluorobenzyl bromide.

Separation and detection are accomplished by capillary gas

chromatography electron-capture negative ion-mass spectrometry

(1,2). Quantitation is based on analysis in the selected ion-monitoring

mode by using 13 stable isotope-labeled internal standards.

(Lagerstedt SA, Hinrichs DR, Batt SM, et al: Quantitative determination

of plasma C8-C26 total fatty acids for the biochemical diagnosis of

nutritional and metabolic disorders. Mol Genet Metab 2001;73:38-45)

Performing Laboratory Location

Rochester

Key