|Values are valid only on day of printing.|
Testing is performed on a Roche Cobas. The Roche TSH/thyrotropin receptor antibody (TSHR Ab) assay is a competitive assay using electrochemiluminescence detection. Patient specimen is treated with a reagent buffer consisting of a preformed immunocomplex of solubilized porcine TSH receptor and biotinylated antiporcine TSH receptor mouse monoclonal antibody. TSHR Ab in patient's serum are allowed to interact with the TSH receptor complex. After addition of streptavidin-coated microparticles and a human thyroid-stimulating monoclonal autoantibody (M22) labeled with a ruthenium complex, bound TSHR Ab are detected by their ability to inhibit the binding of labeled M22. The entire complex becomes bound to the solid phase via interaction of biotin and streptavidin. This reaction mixture is aspirated into measuring cell where the bound microparticles are captured onto the electrode surface and unbound substances are removed. Voltage is applied to the electrode inducing a chemiluminescent emission, which is then measured against a calibration curve to determine the amount of thyrotropin receptor antibody in the patient specimen.(Package insert: Roche Cobas. Roche Diagnostics, Indianapolis, IN 2010-09, V4)
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