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Test ID: THYRO    
Thyrotropin Receptor Antibody, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Testing is performed on a Roche Cobas. The Roche TSH/thyrotropin receptor antibody (TSHR Ab) assay is a competitive assay using electrochemiluminescence detection. Patient specimen is treated with a reagent buffer consisting of a preformed immunocomplex of solubilized porcine TSH receptor and biotinylated antiporcine TSH receptor mouse monoclonal antibody. TSHR Ab in patient's serum are allowed to interact with the TSH receptor complex. After addition of streptavidin-coated microparticles and a human thyroid-stimulating monoclonal autoantibody (M22) labeled with a ruthenium complex, bound TSHR Ab are detected by their ability to inhibit the binding of labeled M22. The entire complex becomes bound to the solid phase via interaction of biotin and streptavidin. This reaction mixture is aspirated into measuring cell where the bound microparticles are captured onto the electrode surface and unbound substances are removed. Voltage is applied to the electrode inducing a chemiluminescent emission, which is then measured against a calibration curve to determine the amount of thyrotropin receptor antibody in the patient specimen.(Package insert: Roche Cobas. Roche Diagnostics, Indianapolis, IN 2010-09, V4)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday 5 a.m. - 12 a.m., Saturday 6 a.m. - 6 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day / same day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 months

Performing Laboratory Location The location of the laboratory that performs the test

Rochester