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Unit Code 81792:
T-Uptake, Serum

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Method Description

This is a chemiluminometric immunoassay. T3-uptake is measured using

an automated sandwich 2-site chemiluminometric immunoassay

on the Advia Centaur instrument. The specimen is incubated with

acridinium ester (AE)-labeled T3-BGG (bovine gamma globulin) and

unlabeled T3. The unlabeled T3 fills available thyroid-binding sites

in the specimen; and the AE-labeled T3-BGG does not bind to the binding

proteins in the specimen. The AE-labeled T3-BGG and the remaining

unlabeled T3 then compete for binding sites on a monoclonal mouse

anti-T3 antibody in the solid phase. A greater amount of unlabeled T3

bound to the binding proteins in the specimen results in more T3-BGG-AE

binding to the monoclonal antibody, an indication of a greater amount of

unsaturated binding proteins. A direct relationship exists between the

concentration of unsaturated binding proteins in a specimen and the relative

light units (RLU's) detected by the system.  (Package insert: Bayer Advia:

Centaur TUP, 2004-03. Bayer Diagnostics, East Walpole, MA 02032)

Performing Laboratory Location

Rochester

Key