|Values are valid only on day of printing.|
Serum proteins are separated in an electric field according to their size, shape, and electric charge. The separation is performed on agarose gels. The proteins are visualized by staining with acid blue and the intensity of staining is quantitated by densitometry (Helena Quick Scan 2000). Multiplying by the serum total protein converts the percentage of protein in each fraction into serum concentration.(Package insert: Helena SPIFE 3000 Instruction Manual and Helena SPIFE SPE Vis Gel 2001)
Immunofixation is performed with Sebia reagent sets and are specific for gamma, alpha, mu, kappa, and lambda immunoglobulin heavy and light chains. If a monoclonal light chain is detected in the absence of an associated monoclonal heavy chain, an immunofixation electrophoresis (IFE) specific for delta and epsilon chains is performed.(Katzmann JA, Kyle RA: Chapter 10: Immunochemical characterization of immunoglobulins in serum, urine, and cerebrospinal fluid. In Manual of Molecular and Clinical Laboratory Immunology. Seventh edition. Edited by B Detrick, RG Hamilton, JD Folds. Washington DC. ASM Press, 2006, pp 88-100)
Free Light Chains:
The quantitation of free light chain (FLC) by nephelometry uses FLC antisera from The Binding Site, Ltd., and is performed on the Siemens Nephelometer II.(Bradwell AR, Carr-Smith HD, Mead GP, et al: Highly sensitive, automated immunoassay for immunoglobulin free light chains in serum and urine. Clin Chem 2001;47:673-80)
Monday through Saturday; 2 p.m.