Test ID: VANCR
Vancomycin, Random, Serum
Method Description
Describes how the test is performed and provides a method-specific reference
Enzyme-multiplied immunoassay technique (EMIT) performed using
the Olympus analyzer. EMIT offers an alternative to the traditional
spectroscopic and chromatographic method for quantitating blood
concentrations of drugs. The technique for drugs is based upon an
enzymatic assay for glucose-6-phosphate dehydrogenase, using
spectral properties at 340 nm, in which the reduction of nicotinamide
adenine dinucleotide (NAD) substrate is monitored. The basis of the
drug detection technique is an immunological reaction between the
drug and a specific antibody. The reagent contains the enzyme
(glucose-6-phosphate dehydrogenase) to which the drug is
Covalently bound and an antibody specific to the drug. The antibody
binds most of the drug-bound enzyme, rendering the enzyme inactive.
This results in a baseline enzymatic activity. In the presence of free
drug, antibody equilibrates between free drug and enzyme-bound
drug leaving some of the drug-bound enzyme uncomplexed and able
to catalyze the reaction. If more free drug is introduced, either as
standard or sample, then competition for the antibody takes place
between the drug in the sample and the drug attached to the enzyme.
This results in more drug-bound enzyme being left uncomplexed and
able to catalyze the enzyme reaction at a greater rate as compared
to the baseline activity. The observed enzyme activity increases
with the amount of total free drug in the sample. (Moyer TP:
Therapeutic drug monitoring. In Tietz Textbook of Clinical Chemistry,
4th edition. Edited by CA Burtis, ER Ashwood. Philadelphia, WB
Saunders Company, 2005, pp 1237-1285)
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Sunday; Varies


