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Unit Code 81749:
Vancomycin-Random

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Method Description

Enzyme multiplied immunoassay technique (EMIT) is performed using

the Olympus analyzer. EMIT offers an alternative to the traditional

spectroscopic and chromatographic method for quantitating blood

concentrations of drugs. The technique for drugs is based upon an

enzymatic assay for glucose-6-phosphate dehydrogenase, using

spectral properties at 340 nm, in which the reduction of nicotinamide

adenine dinucleotide (NAD) substrate is monitored. The basis of the

drug detection technique is an immunological reaction between the

drug and a specific antibody. The reagent contains the enzyme

(glucose-6-phosphate dehydrogenase) to which the drug is covalently

bound and an antibody specific to the drug. The antibody binds most of

the drug-bound enzyme, rendering the enzyme inactive. This results in

a baseline enzymatic activity. In the presence of free drug, antibody

equilibrates between free drug and enzyme-bound drug leaving some

of the drug-bound enzyme uncomplexed and able to catalyze the

reaction. If more free drug is introduced, either as standard or sample,

then competition for the antibody takes place between the drug in the

sample and the drug attached to the enzyme. This results in more drug-

bound enzyme being left uncomplexed and able to catalyze the enzyme

reaction at a greater rate as compared to the baseline activity. The

observed enzyme activity increases with the amount of total free drug

in the sample. (Moyer TP:  Therapeutic drug monitoring. In Tietz

Textbook of Clinical Chemistry, 4th edition. Edited by CA Burtis,

ER Ashwood. Philadelphia, WB Saunders Company, 2005,

pp 1237-1285)

Performing Laboratory Location

Rochester

Key