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Test ID: PNRP    
Pneumocystis jiroveci, Molecular Detection, PCR

Method Description Describes how the test is performed and provides a method-specific reference

Bronchoalveolar lavage fluid and sputum are liquified using N-acetyl-L-cysteine. Following liquification, cells are pelleted by centrifugation and resuspended in S.T.A.R. buffer (Roche). Nucleic acids are extracted using the MagNA Pure LC Instrument (Roche). The extract is then transferred to individual self-contained cuvettes for amplification using the LightCycler real-time PCR platform (Roche). The LightCycler is an automated instrument that amplifies and monitors the development of target nucleic acid (amplicon) after each cycle of PCR. The detection of amplicon is based on fluorescence resonance energy transfer (FRET), which utilizes hybridization probes. The presence of the specific organism nucleic acid is confirmed by performing a melting curve analysis of the amplicon. (Arcenas RC, Uhl JR, Buckwalter SP, et al., A Real-Time PCR Assay for Detection of Pneumocystis from Bronchoalveolar Lavage Fluid. Diagn Microbiol Infect Dis, 2006, 54:169-175)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

1 day

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 to 14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester