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Unit Code 81596:
Glutamic Acid Decarboxylase (GAD65) Antibody Assay, Serum

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Method Description

(125)I-labeled recombinant human GAD65 is incubated with the

patient's diluted serum. Antihuman IgG and IgM are then added to

form an immunoprecipitate. After washing the precipitated immune

complexes, specific antibodies are detected by counting gamma-

emission from the pellet's bound (125)I-GAD65. (Walikonis JE,

Lennon VA:  Radioimmunassay for glutamic acid decarboxylase

[GAD65] autoantibodies as a diagnostic aid for stiff-man syndrome

and a correlate of susceptibility to type 1 diabetes mellitus. Mayo

Clin Proc 1998 December;73[12]:1161-1166)

Performing Laboratory Location

Rochester

Key