(125)I-labeled recombinant human glutamic acid decarboxylase (GAD65) is incubated with the patient's diluted serum. Antihuman IgG and IgM are then added to form an immunoprecipitate. After washing the precipitated immune complexes, specific antibodies are detected by counting gamma-emission from the pellet's bound (125)I-GAD65.(Walikonis JE, Lennon VA: Radioimmunassay for glutamic acid decarboxylase [GAD65] autoantibodies as a diagnostic aid for stiff-man syndrome and a correlate of susceptibility to type 1 diabetes mellitus. Mayo Clin Proc 1998 December;73:1161-1166)
Sunday through Thursday; 10 p.m.
2 days/negative 3 days/positive