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Unit Code 8159:
Immunoglobulin E (IgE), Serum

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Method Description

Anti-IgE, covalently coupled to ImmunoCAP, reacts with the IgE in

a serum specimen. After washing, enzyme-labeled anti-IgE antibodies

are added to form a complex. After incubation, unbound enzyme-

labeled anti-IgE is washed away and the bound complex is incubated

with a developing agent. After stopping the reaction, fluorescence

in the well is measured. The fluorescence is directly proportional

to the concentration of IgE in the test specimen. (Package insert:

Phadia CAP System IgE FEIA. 52-1939-07, issued April 1998,

revised October 1999)

Performing Laboratory Location

Rochester

Key