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Test ID: CYCL    
Cyclospora Stain

Method Description Describes how the test is performed and provides a method-specific reference

Safranin stain of stool concentrate. Fecal specimens may be submitted fresh or preserved in formalin or ECOFIX. Prepare a thin smear of the fecal material (preserved fecal specimens or fecal concentrate) to be stained. Air dry the smear. Place in acidic alcohol (3%) for 5 minutes. Wash slides with cold tap water and place slides in coplin jar containing safranin (1%) solution in acidified water (pH 6.5) and microwave at full power (650w) for 1 minute. Wash off excess stain with tap water. Place the slides in a coplin jar containing an aqueous solution of either 1% methylene blue or 1% malachite green and let stand for 1 minute. Rinse gently with tap water, let dry and mount with a number 1 coverslip. The oocysts of Cyclospora will stain a brilliant reddish orange. (Visvesvara GS, Moura H, Kovacs-Nace E, et al: Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating. J Clin Microbiol 1997;35:730-733)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Saturday; 12 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester