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Mumps virus antibodies are detected by the indirect immunofluorescence procedure. Spinal fluid from the patient is diluted and placed in wells of substrate slides containing mumps virus-infected cells. A fluorescent antibody conjugate is then allowed to react with the virus-infected cells.(Brown GC, Baublis JV, O'Leary TP: Development and duration of mumps fluorescent antibodies in various immunoglobulin fractions of human serum. J Immunol 1970;104:86-94)
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