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Unit Code 81405:
Dehydroepiandrosterone (DHEA), Serum

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Method Description

Deuterated stable isotope (d[2]-DHEA) is added to a 0.4 mL serum

sample as internal standard. The DHEA and internal standard are

extracted from the sample by solid-phase extraction. This is followed

by conventional liquid chromatography on a Cohesive LX4 System

and analysis on a tandem mass spectrometer (MS/MS) equipped

with a heated nebulizer ion source. (Soldin OP, Guo T, Weiderpass

E, et al:  Steroid hormone levels in pregnancy and 1 year postpartum

using isotope dilution tandem mass spectrometry. Fertil Steril 2005

Sept:84[3]:701-710)

Performing Laboratory Location

Rochester

Key