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Unit Code 81369:
Fatty Acid Profile, Peroxisomal (C22-C26), Serum

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Method Description

Acidic hydrolysis is followed by basic hydrolysis and reacidification.

Hexane extraction then proceeds to derivatization with

pentafluorobenzyl bromide (PFB). Separation and detection of PFB-esters

is accomplished by capillary gaschromatograph-mass spectrometry

(GC/MS) using electron capture ionization and selected negative ion

monitoring. Quantitation is enhanced by the use of stable isotope-labeled

internal standards. (Stellard F, ten Brink HJ, Kok RM, et al: Stable isotope

dilution analysis of very long chain fatty acids in plasma, urine and

amniotic fluid by electron capture negative ion mass fragmentography.

Clin Chim Acta1990;192:133-144)

Performing Laboratory Location

Rochester

Key