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Unit Code 81249:
Acylglycines, Quantitative, Urine

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Method Description

Urine volumes equivalent to 0.25 mg to 0.50 mg of creatinine are spiked

with the mixture of labeled internal standards, allowed to equilibrate,

acidified, then extracted with ethyl acetate. After evaporation, the

dry residue is  derivatized to butyl esters. Specimens are analyzed

by capillary gas GC-MS selected ion monitoring using ammonia

chemical ionization and a stable isotope dilution method. (Rinaldo

P, O'Shea JJ, Welch RD, Tanaka K:  Stable isotope dilution

analysis of n-hexanoylglycine, 3-phenylpropionylglycine and

suberlglycine in human urine using chemical ionization gas

chromatography-mass spectrometry selected ion monitoring.  

Biomed Environ Mass Spectrom 1989;18:471-477)

Performing Laboratory Location

Rochester

Key