|Values are valid only on day of printing.|
The Legionella kit is designed to detect IgG/A/M class antibodies to Legionella pneumophila in human sera. The test procedure involves 3 incubation steps:
1. Test sera (properly diluted) are incubated in multiwells coated with an inactivated, solubilized cocktail of Legionella pneumophila groups 1-6 bacteria (antigen). Legionella specific IgG, IgM, or IgA antibodies in the specimen will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components.
2. Peroxidase conjugated goat antihuman IgG/A/M is added to the wells and the plate is incubated. The conjugate will react with antibody immobilized on the solid phase in step 1. The wells are washed to remove unreacted conjugate.
3. The multiwells containing immobilized peroxidase conjugate are incubated with peroxidase substrate solution. Hydrolysis of the substrate by peroxidase produces a color change. After a period of time, the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the test specimen.(Package insert: Legionella IgG, A, M ELISA II, Wampole Laboratories, Princeton, NJ 2005-016-16)
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