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Unit Code 81128:
Babesia microti IgG Antibodies, Serum

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Method Description

This immunoflourescence assay (IFA) detects antibodies against

Babesia microti. The patient's serum is diluted 1:64 and 20 uL is

placed in microscopic slide wells which have been coated with

Babesia microti infected RBCs from Syrian hamsters. After incubation,

the slides are washed and a fluorescein isothiocyanate conjugate

is added to each well. The slides are then read using a fluorescence

microscope and significant fluorescent staining of intraerythrocytic

organisms constitutes a positive reaction. (Krause PJ, Telford SR III,

Ryan R, et al:  Diagnosis of babesiosis:  Evaluation of a serologic

test for the detection of Babesia microti antibody. J Infect Dis 1994;

169:923-926)

Performing Laboratory Location

Rochester

Key