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C3 complement activity is measured by mixing patient serum with
a C3-deficient serum. The lytic activity of the serum mixture is tested
against sensitized, labeled liposomes. If lysis occurs, the patient
serum must be the source of the C3. The target liposomes are a
commercial reagent (WAKO total complement CH[50]), and the
assay is performed on a Hitachi 912. (Unpublished Mayo information
Yamamoto S, Kubotsu K, Masaaki K, et al: Automated homogeneous
liposome-based assay system for total complement activity.
Clin Chem 1995;41:586-590)

