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Unit Code 81090:
C3 Complement, Functional, Serum

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Method Description

C3 complement activity is measured by mixing patient serum with

a C3-deficient serum. The lytic activity of the serum mixture is tested

against sensitized, labeled liposomes. If lysis occurs, the patient

serum must be the source of the C3. The target liposomes are a

commercial reagent (WAKO total complement CH[50]), and the

assay is performed on a Hitachi 912. (Unpublished Mayo information

Yamamoto S, Kubotsu K, Masaaki K, et al: Automated homogeneous

liposome-based assay system for total complement  activity.

Clin Chem 1995;41:586-590)

Performing Laboratory Location

Rochester

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