Aneuploidy Detection, Products of Conception (POC), FISH
Method Description Describes how the test is performed and provides a method-specific reference
This FISH test uses commercially available chromosome-specific fluorescent-labeled DNA probes for FISH. The 3 probe combinations utilized include: centromere probes for chromosome X (DXZ1) in green, Y (DYZ3) in orange, and 18 (D18Z1) in aqua; locus-specific probes for chromosomes 13 at 13q14 in green and 21 (D21S259, D21S341, D21S342) at 21q22.13-q22.2 in orange; a centromere probes for chromosome 16 (D16Z3) in aqua and chromosome 15 (D15Z4) in orange and a locus-specific probe for chromosome 22 (BCR) at 22q11.2 in green. The above centromere probes hybridize to the alpha-satellite DNA regions.
Formalin-fixed, paraffin-embedded tissues are cut at 5 microns and mounted on positively charged glass slides. Two slides are prepared with 2 hybridization sites per slide. Each probe set is hybridized to a separate hybridization site. For each probe, 200 interphase nuclei are scored and results for each abnormal probe(s) are expressed as percent abnormal nuclei. Aneuploidy of chromosomes 13, 15, 16, 18, 21, 22, X, and/or Y is reported. (Unpublished Mayo method)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m. CST.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test